Genetic determinants of <i>Mycobacterium tuberculosis</i> adaptation and drug efficacy during stationary phase growth.

ABSTRACT: The adaptation of Mycobacterium tuberculosis (Mtb) to a slowly growing or nongrowing state in growth-limited conditions plays a crucial role for drug tolerance. Although the mechanisms of Mtb adaptation under growth-limited conditions have been extensively studied, it remains unclear to what extent the cellular processes necessary to sustain nongrowing state affect drug efficacy. To investigate this, we performed a genome-wide transposon mutant screen, which allowed parallel identification of the genes that influence bacterial fitness and drug efficacy during the stationary phase. Our analysis revealed that genes encoding the SOS response, membrane phospholipid biosynthesis, proteasomal protein degradation, and cell wall remodeling critically determine Mtb fitness in both stationary

SUBMITTER: Shi X-J 

PROVIDER: S-EPMC12403877 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature