The role of ASS1 in mouse embryonic stem cell differentiation into mesendoderm lineages.

ABSTRACT:

Background

Argininosuccinate synthase (ASS1) is a pivotal enzyme involved in the urea cycle, playing a crucial role in aspartate catabolism, arginine and nitric oxide biosynthesis. These biological processes are crucial for the growth and development of mammals. However, the functions of urea cycle-related genes in mouse embryonic stem cells (mESCs) remain largely unclear. Here, we investigated the impact of ASS1 knockout on the mESCs pluripotency and its role in determining cell fate.

Methods

ASS1 was knocked out in mESCs using CRISPR-Cas9. Changes in pluripotency post-knockout were analyzed via immunofluorescence, Western blotting, differentiation assays, and chimera formation. Cardiomyocyte differentiation assays evaluated the impact on cardiac lineage specification. RNA

SUBMITTER: Zhao G 

PROVIDER: S-EPMC12403944 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature