Statistical analysis of multi-endpoint phenotypic screening increases sensitivity of planarian neurotoxicity testing.

ABSTRACT: There is an urgent need for high-throughput screening (HTS) models to replace, refine, and/or reduce ("3Rs") vertebrate toxicity testing. Replacing in vivo animal studies is challenging for neurotoxicity and developmental neurotoxicity (DNT) where the functional relevancy of adverse outcomes needs to be assessed on the whole organism. We previously screened the NTP 87-compound library (NTP87), consisting of known and suspected developmental neurotoxicants, and showed that planarian HTS can identify known (developmental-) neurotoxicants. Because analysis methods can impact screening results and our original analysis used lowest observed effect level (LOEL) only, we hypothesized that use of state-of-the-art statistical analysis would increase sensitivity of planarian HTS to identify neurotox

SUBMITTER: Ireland D 

PROVIDER: S-EPMC12404179 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature