The MIR157-SPL15 module regulates flowering and inflorescence development in Arabidopsis thaliana under short days and in Arabis alpina.

ABSTRACT: The plant life cycle progresses through distinct phases defined by the morphology of the organs formed on the shoot. In Arabidopsis, age-dependent reduction in the related microRNAs miR156 and miR157 controls transitions from juvenile to adult vegetative phase and from adult to reproductive phase. However, whether these miRNA isoforms have specific contributions remains unclear. To compare their roles, we used Trans-kingdom, rapid, affordable Purification of RISCs (TraPR) for small RNA sequencing, CRISPR-Cas9, and confocal imaging. We show that in shoot apices, levels of miR156 in RNA-induced silencing complexes (RISCs) decline more rapidly than those of miR157, so that miR157 is more abundant than miR156 in RISCs of older plants undergoing floral transition and inflorescence development.

SUBMITTER: Roggen A 

PROVIDER: S-EPMC12404414 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature