Decellularized rat brain extracellular matrix effectively induces the dopaminergic differentiation of human adipose-derived stem cells.

ABSTRACT: The extracellular matrix (ECM) plays essential roles in regulating various aspects of nervous system development. The ECM can be obtained through decellularization techniques, which preserve the native structure of tissue while removing cells and genetic material. Despite recent advancements in decellularization methods, removing cells from brain tissue remains challenging due to its delicate mechanical structure. Moreover, previous studies have not specifically evaluated the impacts of decellularized brain ECM on dopaminergic specification of stem cells. Here, we decellularized rat brain sections using a combination of chemical and enzymatic factors. Successful decellularization of sections was confirmed by DAPI, Haematoxylin and Eosin and Masson's trichrome staining, laminin immunostaini

SUBMITTER: Faghih H 

PROVIDER: S-EPMC12404474 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature