Deciphering the role of the lncRNA TRIBAL in hepatocyte models.

ABSTRACT: We recently reported that the long non-coding RNA TRIBAL/TRIB1AL was required to sustain key hepatocyte functions. Here, we identify HepaRG cells as a model for studying TRIBAL and provide additional validation and functional insights. In contrast to HepG2 and HuH-7 cells, differentiated HepaRG cells showed similarities to primary hepatocytes in response to TRIBAL suppression. TRIBAL suppression was associated with reduced HNF4A and MLXIPL abundance in hepatocytes and HepaRG cells. TRIBAL targeting using a panel of cognate antisense oligonucleotides confirmed specificity. A comparison of TRIBAL-suppressed hepatocyte and HepaRG transcriptomics identified extensive functional overlap. Biological ontologies associated with key hepatic metabolic functions were predicted to be inhibited in both

SUBMITTER: Soubeyrand S 

PROVIDER: S-EPMC12404505 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature