Determination of epidemiological cut-off values for Narasin, Salinomycin, Lasalocid and Monensin in Enterococcus faecium.

ABSTRACT:

Objectives

To establish epidemiological cut-off values (ECOFFs) for the polyether ionophores narasin, salinomycin, lasalocid and monensin in Enterococcus faecium.

Methods

MICs were measured using the broth microdilution method according to ISO 20776-1 (2019). Method validation involved ≥10 replicates of antimicrobial susceptibility testing of Enterococcus faecalis ATCC 29212. A total of 182 E. faecium isolates from various sources were tested in five European laboratories. The ECOFFinder tool from EUCAST was used to establish the ECOFFs for 122 WT isolates, verified by PCR or WGS.

Results

Method validation showed consistency, with acceptable variation within ±1 2-fold dilution. The ECOFF for narasin was 0.5 mg/L, considerably below the current EUCAST ECOFF for E. faec

SUBMITTER: Ferrando ML 

PROVIDER: S-EPMC12404726 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature