Increase in Angiogenesis and Vascularization in Patient-Derived Endometriosis Tissue: Insights from a 3D In Vivo Model.

ABSTRACT:

Aim

Endometriosis is a gynecological disorder characterized by endometrial-like tissue outside the uterus. This study evaluates the vascularization and proliferation of human endometriosis and endometrium tissues engrafted onto the chorioallantoic membrane of chicken embryos using immunohistochemistry and laser speckle contrast analysis imaging. For the assessment of clinical relevance, a comparison between laboratory and clinical data was performed.

Material and methods

Tissue samples from 10 patients categorized by #Enzian scores and undergoing endometriosis surgery were investigated in the chorioallantoic membrane model. Hematoxylin-eosin staining and immunohistochemical markers, including CD10, cytokeratin, Ki67, and Caspase-3, assessed cellular structures, proliferation

SUBMITTER: Scherer P 

PROVIDER: S-EPMC12404789 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature