Cell type-specific in vivo proteomes with a multicopy mutant methionyl tRNA synthetase mouse line.

ABSTRACT: The functional diversity of cells is driven by the different proteins they express. While improvements in protein labeling techniques have enabled the measurement of proteomes with increased sensitivity, measuring cell type-specific proteomes in vivo remains challenging. One of the most useful pipelines is bio-orthogonal noncanonical amino acid tagging (BONCAT) with the MetRS* system, consisting of a transgenic mouse line expressing a mutant methionyl-tRNA synthetase (MetRS*) controlled by Cre recombinase expression. This system allows cell type-specific labeling of proteins with a noncanonical amino acid (azidonorleucine, ANL), which can be subsequently conjugated to affinity or fluorescent tags using click chemistry. Click-modified proteins can then be visualized, purified and identified

SUBMITTER: Alvarez-Pardo R 

PROVIDER: S-EPMC12404990 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature