Mollugin reacts with phenol thiol but not produce modification on cysteine discovered by a phenol thiol probe.

ABSTRACT: Electrophilic compounds from natural products (NPs) and metabolites can covalently modify the cysteines of target proteins to induce biological activities. To facilitate the discovery of novel NPs and metabolites, chemical probes with various thiol groups-mimicking the reactivity of cysteine-have been developed. These probes are designed to react with electrophilic groups of NPs and metabolites in an electrophilic addition mechanism, with the resulting adducts having molecular masses which equal to the sum of the probe and the target compound. This principle has been fundamental to analyzing mass spectrometry (MS) data and calculating the exact molecular weights of the target compound. In this study, we report a phenol thiol probe initially designed to mimic cysteine reacts with Mollugin a

SUBMITTER: Liu T 

PROVIDER: S-EPMC12405156 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature