SFB flagellin mediates cell adhesion, endocytosis and immune regulation in germ-free mice.

ABSTRACT:

Introduction

Segmented filamentous bacteria (SFB) colonization dynamics are crucial for host immune regulation. Given this, the present study specifically examined the functions of SFB flagellin in bacterial adhesion, cellular internalization, and immune modulation.

Methods

Lactococcus lactis and Escherichia coli were engineered to express murine and rat SFB flagellin genes. Subsequent in vitro co-culture experiments with intestinal epithelial cell lines (MODE-K and IEC-18) and germ-free mouse colonization assays were conducted. Bacterial counts, immunohistochemical analysis, the AAM-ISO-G1 and QAM-TH17-1 microarray systems, RNA sequencing and molecular docking were employed to assess the outcomes in this study.

Results and discussion

The results

SUBMITTER: Chen H 

PROVIDER: S-EPMC12405183 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature