Transcriptional reprogramming of Novacetimonas hansenii SI1 during growth on glycerol.

ABSTRACT: Bacterial nanocellulose (BNC) is a valuable biopolymer with immense potential in various sectors of biotechnology. However, large-scale production is hindered by low yields and high costs. Glycerol is an inexpensive and widely available carbon source for BNC biosynthesis, as it is a by-product of the biofuel industry. Compared to glucose, this polyol enhances BNC yields of Novacetimonas hansenii SI1 and related strains. This study investigates transcriptomic changes in N. hansenii SI1 after switching from glucose to glycerol using RNA-seq. The results reveal metabolic reprogramming, including upregulation of genes involved in glycerol uptake and catabolism, gluconeogenesis, the pentose phosphate pathway, and the Entner-Doudoroff pathway. Glycerol metabolism induces oxidative stress, eviden

SUBMITTER: Wlazlak M 

PROVIDER: S-EPMC12405394 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature