Isowighteone attenuates vascular calcification by targeting HSP90AA1-mediated PI3K-Akt pathway and suppressing osteogenic gene expression.

ABSTRACT:

Background

Isowighteone, an isoflavonoid compound derived from Ficus hispida L.f. (F. hispida, Moraceae), has demonstrated significant anti-inflammatory properties in prior studies. However, its anti-inflammatory role in vascular calcification is unclear.

Object

We investigated the efficacy of isowighteone in the treatment of vascular calcification, explored its potential mechanism, and determined whether isowighteone is a safe and effective treatment.

Methods

In this study, we isolated three natural compounds and evaluated their efficacy using in vitro calcification models through CCK-8 assays, Alizarin Red staining, and calcium quantification. The key targets of Isowighteone were identified via network pharmacology and molecular docking analyses

SUBMITTER: Mo Y 

PROVIDER: S-EPMC12405412 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature