Multi-omics approaches reveal the mechanisms underlying the interaction between cyst fluid of <i>Echinococcus granulosus</i> and host immune cells.

ABSTRACT:

Background

Echinococcus granulosus cyst fluid (EgCF) is a complex mixture of parasite's containing a variety of antigens. Th9 cells are a newly reported subpopulation of Th cells whose primary function is to secrete IL-9 and exert biological effects. Research on whether antigens in the vesicle fluid can evade the host immune response by increasing IL-9 secretion is limited.

Methods

The effects of EgCF on lymphocyte function in mice were evaluated using CCK-8 and flow cytometry for apoptosis. The effect of EgCF on CD4⁺IL-9⁺T cell differentiation was reflected by flow cytometry. The expression of TGF-β, IL-4, PU.1, IRF4 and IL-9 was detected by WB, qRT-PCR and ELISA under the influence of varying concentrations of EgCF. Analysis of differential met

SUBMITTER: He R 

PROVIDER: S-EPMC12405415 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature