Endogenous ligands of bovine FFAR2/GPR43 display distinct pharmacological properties.

ABSTRACT:

Introduction

Free fatty acids (FFAs) have been identified as ligands for members of the G protein-coupled receptor (GPCR) family, called free fatty acid receptors (FFARs). Among these receptors, there is a particular interest in the physiological roles of FFAR2 and its potential use as a therapeutic target for various health disorders. Despite great progress in other species, pharmacological properties of the bovine FFAR2 (bFFAR2) are not fully understood. The aim of the current study was to evaluate how a selection of FFAs (C2:0 to C8:0, and branched FFAs) activate and regulate bFFAR2 signaling.

Methods

We used HEK293A cells and BRET assays to measure Gαi/Gαq coupling and signaling, β-arrestin 2 recruitment, and receptor internalization/trafficking. SRE and NFAT-RE dependen

SUBMITTER: Michelotti TC 

PROVIDER: S-EPMC12405480 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature