Single cell RNA seq reveals the pro-regenerative phenotype of thrombospondin-2 deficient dermal fibroblasts.

ABSTRACT: Fibroblasts display complex functions linked to distinct gene expression profiles. These profiles influence matrix production, cell communication, and tissue development and repair. Thrombospondin-2 (TSP2), produced by fibroblasts, is a potent angiogenesis inhibitor and negatively associated with tissue repair. Single-cell RNA sequencing (scRNA-seq) on WT and TSP2 KO skin fibroblasts demonstrate distinct cell heterogeneity. Specifically, we found an enrichment of Sox10 + multipotent progenitor cells, identified as Schwann precursor cells, in TSP2 KO fibroblasts, while fibrosis-related subpopulations decreased. Immunostaining of mouse back skin wounds and cells validated the increase of this Sox10 + population. Furthermore, in silico analysis suggested enhanced PDGF-β-mediated pro-survival

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12405501 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature