Development and validation of a methylation-specific droplet digital PCR multiplex for lung cancer detection.

ABSTRACT: Biomarkers are increasingly used in cancer management, including lung cancer. The use of circulating tumour DNA (ctDNA) detection has attracted significant interest as a non-invasive, highly specific, and sensitive strategy. In this study, we developed and validated a methylation-specific droplet digital PCR (ddPCR) multiplex assay with five tumour-specific methylation markers identified by in silico analysis for lung cancer detection across various clinical settings. The performance of the ddPCR multiplex was validated in tissue and plasma in cohorts of healthy controls and patients with both non-metastatic and metastatic disease by examining sensitivity, specificity, and marker dynamics. Furthermore, two different cut-off methods to determine ctDNA-status and their effects on sensitivity

SUBMITTER: Jacobsen CM 

PROVIDER: S-EPMC12405542 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature