Cancer-associated fibroblasts are associated with neo-adjuvant treatment response in oesophageal adenocarcinoma.

ABSTRACT:

Background

Neoadjuvant treatment (NAT) in oesophageal adenocarcinoma (EAC) is characterised by differential responses between patients and treatment modalities. The components of the tumour microenvironment (TME) that contribute to this are unknown. We explored this, focusing on cancer-associated fibroblasts (CAF) an abundant TME component.

Methods

We performed histopathologic, single-cell RNA sequencing and transcriptomic analysis on 26 patients, stratified by pathological response to NAT, and validated a prognostic model in genomic consortia cohorts. Patient-derived cells were used to model CAF phenotypes in vitro.

Results

We observed changes in the TME in response to the NAT received. Specific changes in fibroblasts correlated with treatment response and altered ge

SUBMITTER: Walker RC 

PROVIDER: S-EPMC12405553 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature