Liquid Chromatographic and Mass Spectrometric Methods for Quantitative Proteomic Analysis from Single-Cell and Nanogram-Level Samples.

ABSTRACT: Liquid chromatography (LC) and mass spectrometry (MS) are two critical components in proteomics. Advances in methods for both LC and MS have significantly enhanced protein identification and quantifications of limited amounts of proteins, particularly at the picogram-to-nanogram level of proteins. In this study, we explored various LC conditions and MS platforms to optimize protein identification and quantification using data-independent acquisition (DIA). Our investigation focused on evaluating the sensitivity for protein identification, reproducibility of quantification, and robustness across multiple models, specifically focused on analyzing proteins at pico- to nanogram levels, with an emphasis on single-cell proteomics. We further applied our approach for the proteomic analysis of HeL

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12405731 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature