Nephelometry as a simple tool to monitor the disassembly of polymeric nanoparticles.

ABSTRACT: Polymer-based nanoparticles (PNPs) find wide application in e.g. material and pharmaceutical sciences. In order to determine the quality of produced PNPs and understand their function, analytical characterization is essential. Recently, two-dimensional liquid chromatography (2D-LC) has been introduced as an effective means to probe multiple PNP properties, such as size and composition, in an integrated manner. Such approaches involve transformation of PNPs into their constituents between the two LC dimensions by introducing an organic solvent. Employing light scattering, which is mainly caused by intact PNPs, this paper presents nephelometry as a straightforward technique to assess the percentage of organic solvent needed to achieve full disintegration of PNPs. Empty and dye-loaded

SUBMITTER: Verduin J 

PROVIDER: S-EPMC12405835 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature