Highly replicated experiments studying complex genotypes using nested DNA barcodes.

ABSTRACT: Many biological experiments involve studying the differences caused by genetic modifications, including genotypes composed of modifications at more than 1 locus. However, as the genotypes increase in number and complexity, it becomes a major challenge to independently generate and track the necessary number of biological replicate samples. A major development in genetic studies of large numbers of genotypes has been the use of barcode tracking. Inspired by such high-throughput studies, we developed a barcode-based method to track large numbers of independent replicates of a small number of combinatorial genotypes in a pooled format, enabling robust detection of subtle phenotypic differences. To construct a plasmid library of combinatorial genotypes, we utilized a nested serial cloning proc

SUBMITTER: Monge M 

PROVIDER: S-EPMC12405881 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature