Phosphatidylinositol 3-Kinase Signaling Enhances Intestinal Crypt Epithelial Cell Recovery after Radiation.

ABSTRACT: Intestinal stem cell (ISC) signaling maintains the balance of self-renewal and differentiation. Herein, the role of phosphatidylinositol 3-kinase (PI3K) signaling in ISC responses to radiation was interrogated using Villin-Cre pik3r1^lox/lox (p85^ΔIEC) mice and p85α-deficient human enteroids (shp85α). Lethal whole-body irradiation in mice was performed to monitor PI3K-mediated survival responses. Rectal biopsies from patients with radiation proctitis were examined by immunohistochemistry for the PI3K/Akt- and Wnt-target survivin. The intestinal epithelial cells (IECs) from p85^ΔIEC mice showed increased protein levels of phosphorylated phosphatase and tensin homolog, phosphorylated Akt^Ser473, survivin, cyclin D1, and ρ-β-catenin^Ser552, as

SUBMITTER: Lynch EB 

PROVIDER: S-EPMC12405924 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature