Levo-Tryptophan Promotes Osteogenesis Through Calcium-Sensing Receptor.

ABSTRACT: Previous studies reported the pro-osteogenic ability of L-Tryptophan (L-Trp) and Calcium-Sensing RCeceptor (CaSR) respectively. Recent researchers found L-Trp could activate CaSR. Therefore, this study investigated the osteogenic mechanisms of L-Trp through CaSR activation. Using in vivo and in vitro models, we evaluated L-Trp's effects on bone formation and osteoblast activity. Levo-Trp solution was injected into the temporomandibular joint of 3-week-old mice, and the mandibular development was observed by Micro-CT at 6 weeks of age. The pre-osteoblast cell line MC3T3-E1 cells were stimulated by L-Trp in vitro, and their proliferation, migration, and osteogenic ability were detected by CCK8 assay, alizarin red staining, etc. Transcriptome sequencing was used to investigate the underlying

SUBMITTER: Li P 

PROVIDER: S-EPMC12406082 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature