Circular RNA hsa_circ_0099188 regulates inducible nitric oxide synthase and chemokine transcription in macrophages by targeting the hsa-miR-381-3p/PPP3CA and hsa-miR-381-3p/KLF4 pathways in response to 4,4'-methylene diphenyl diisocyanate-glutathione conjugate exposure.

ABSTRACT: Workplace exposure to 4,4'-methylene diphenyl diisocyanate (MDI), the most used monomeric diisocyanate, can lead to the development of occupational asthma (OA). However, the molecular mechanisms by which MDI induces OA remain poorly understood. Previous studies have shown that exposure to MDI or MDI-glutathione (GSH) conjugate reduces the levels of endogenous human (hsa)/murine (mmu)-microRNA (miR)-206/381-3p, triggering the activation of calcineurin/nuclear factor of activated T-cells/inducible nitric oxide synthase (NOS2) regulatory axis and Krüppel-like factor 4 (KLF4)/chemokine pathways in macrophages. Circular RNAs (circRNAs) play important roles on miR and miR-mediated functions in the cells. CircRNA hsa_circ_0008726 is induced by MDI-GSH to downregulate endogenous hsa-miR-206-3p in

SUBMITTER: Lin CC 

PROVIDER: S-EPMC12406230 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature