Macrophage derived VEGF regulates macrophage senescence to inhibit radiation-induced dermatitis.

ABSTRACT:

Background

Macrophages are essential for maintaining tissue homeostasis and accelerating the repair processes; however, their functionality can be severely compromised in pathological conditions such as radiation-induced dermatitis. In this study we analyzed the role of macrophage derived Vascular Endothelial Growth Factor (VEGF) on regulation of macrophage senescence and its role on radiation-induced skin damage.

Methods

We used bone marrow-derived macrophages (BMMɸ) isolated from Csf1r-iCre; VEGF^fl/fl (VEGF-null) and wild-type (WT) mice. Macrophages were exposed to oxidative and genotoxic stress using H₂O₂, doxorubicin, and radiation exposure to evaluate senescence. Senescence was assessed via SA-β-Gal staining and expression of senescence-related genes. Additio

SUBMITTER: Chugh RM 

PROVIDER: S-EPMC12406352 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature