Untargeted metabolomics reveals changes in boar sperm and seminal plasma metabolites associated with sexual maturity.

ABSTRACT:

Background

Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity. However, comprehensive metabolomic profiles of boar semen remain uncharacterised. Understanding metabolic alterations in semen during this period is important for optimising reproductive performance in breeding programs. The aim of this study was to characterise the semen metabolome as boars mature, utilising an untargeted metabolomic approach. Semen samples were collected from 15 Duroc boars at three developmental ages: ~ 7 months, 8.5 months, and 10 months. Sperm and seminal plasma were separated and analysed by hydrophilic interaction and reversed-phase liquid chromatography coupled with mass spectrometry to capture a wide range of metabolites.

Res

SUBMITTER: Shrestha A 

PROVIDER: S-EPMC12406428 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature