Biomimetic nanoparticles coated with ScFv-modified macrophage membranes for siRNA delivery to relieve brain metastases of lung cancer.

ABSTRACT:

Background

In China, lung cancer stands as the leading cause of cancer-related deaths, often resulting in brain metastases (BM) that severely compromise patients' quality of life and reduce survival outcomes. The delivery of drugs to the brain is further complicated by the blood-brain barrier (BBB). To address this, we developed EGFR single-chain fragment variable (scFv)-modified macrophage membrane liposomes (scFv-MML) encapsulating LPCAT1 siRNA (scFv-MML@LPCAT1si) as a targeted therapy for non-small cell lung cancer (NSCLC) BM.

Methods

EGFR scFv-pcDNA3.1(-) plasmids were transfected into RAW 264.7 cells to generate RAW 264.7-scFv cells. Macrophage membranes were isolated from these cells and used to coat liposomes (Lip) encapsulating LPCAT1 siRNA via extrusion. The cellula

SUBMITTER: Lu Y 

PROVIDER: S-EPMC12406594 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature