Indolenine-substituted pyrazole derivative 4e inhibits planktonic <i>Staphylococcus lugdunensis</i> growth and biofilm formation by disrupting purine biosynthesis and compromising cell wall and membrane integrity.

ABSTRACT: Staphylococcus lugdunensis is an emerging nosocomial pathogen responsible for biofilm-related infections. Here, we explored the antibacterial and antibiofilm properties of the novel indolenine derivative 4e against S. lugdunensis and investigated its mechanisms of action. Its antibacterial and antibiofilm activities were assessed against oxacillin-resistant S. lugdunensis CGMH-SL131 using in vitro and in vivo models, including human cell lines, Galleria mellonella larvae, and mice. Mechanistic insights were explored via untargeted metabolomics. 4e exhibited bacteriostatic activity against a panel of gram-positive bacteria, with a 1× minimum inhibitory concentration (MIC) of 62.5 µg/mL. Scanning electron microscope observations of cells treated with

SUBMITTER: Hidrosollo JH 

PROVIDER: S-EPMC12406677 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature