Complementary killing activities of <i>Pbunavirus LS1</i> and <i>Bruynoghevirus LUZ24</i> phages on planktonic and sessile <i>Pseudomonas aeruginosa</i> PAO1 derivatives.

ABSTRACT: Four phages active against a representative panel of Pseudomonas aeruginosa strains were chosen with the goal of using them for phage therapy. Two were belonging to the Pbunavirus LS1 species, and two to the Bruynoghevirus LUZ24 species. The receptor of the P. LS1 phage Ab27 had already been characterized as the O-antigen chain of the lipopolysaccharides, whereas no information was available at the onset of this work on the receptor used by the B. LUZ24 phages. We show that this receptor is the surface polysaccharide Psl, an important component of the biofilm matrix. Remarkably, the B. LUZ24 phages were more active against PAO1 in minimal medium compared to rich medium. This was correlated with larger amounts of Psl bound at the bacterial surface d

SUBMITTER: Billaud M 

PROVIDER: S-EPMC12406683 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature