Single-cell spatial transcriptomics unravels the cellular landscape of abdominal aortic aneurysm.

ABSTRACT: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with no effective pharmacological interventions. While single-cell transcriptomics has advanced our understanding of AAA, it lacks spatial context. Here, we employed Seq-Scope, an ultra-high-resolution spatial transcriptomic technology, to decipher the spatial landscape of angiotensin II-induced AAA in Apoe-/- mice. Our analysis revealed the heterogeneity of macrophages, fibroblasts, and smooth muscle cells (SMCs), with specific responses in different layers of the AAA tissue. SMCs in the inner layers showed associations with Mgp-expressing fibroblasts and GPNMB-expressing macrophages, whereas the outer layers had different dominant cell types. Notably, GPNMB-expressing macrophages were concentrated near SMCs in regions

SUBMITTER: Zhao G 

PROVIDER: S-EPMC12406718 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature