Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer.

ABSTRACT: Breast cancer is the most prevalent form of malignant tumor that frequently metastasizes to axillary lymph nodes (LNs). Nonetheless, the precise mechanisms underlying alterations in the tumor microenvironment (TME) in LN metastasis in breast cancer remain poorly understood. Single-cell RNA sequencing of 28 LN samples from 23 patients is performed, and a comprehensive landscape of the entire ecosystem is generated. Ten major cell types are identified, with the subclusters of each major cell type exhibiting diverse characteristics. Furthermore, multiple signatures are collected to evaluate the key components of the subclusters using multi-omics methodologies. This study finds that myCAFs may hasten LN metastasis, and observed a notable increase in APOE+ macrophages and a higher proportion of

SUBMITTER: Xie J 

PROVIDER: S-EPMC12407353 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature