DNA Dynamics in Dual Nanopore Tug-of-War.

ABSTRACT: Solid state nanopores have emerged as powerful tools for single-molecule sensing, yet the rapid uncontrolled translocation of the molecule through the pore remains a key limitation. We have previously demonstrated that an active dual-nanopore system, consisting of two closely spaced pores operated via feedback controlled biasing, shows promise in achieving controlled, slowed-down translocation. Translocation control is achieved via capturing the DNA in a special tug-of-war configuration, whereby opposing electrophoretic forces at each pore are applied to a DNA molecule co-captured at the two pores. Here, we systematically explore translocation physics during DNA tug-of-war focusing on genomically relevant longer dsDNA using a T₄-DNA model (166 kbp). We find that longer molecules

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12407631 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature