Robust fluorescent labeling and tracking of endogenous non-repetitive genomic loci.

ABSTRACT: The spatial organization and dynamics of a genome are central to gene regulation. While a comprehensive understanding of chromatin organization in the human nucleus has been achieved using fixed-cell methods, measuring the dynamics of specific genomic regions over extended periods in individual living cells remains challenging. Here, we present a robust and fully genetically encoded system for fluorescent labeling and long-term tracking of any accessible non-repetitive genomic locus in live human cells using fluorogenic and replenishable nanobody array fusions of the Staphylococcus aureus dCas9, and compact polycistronic single guide (sg)RNAs. First, we characterize the selectivity and photostability of our probes, enabling genome-wide visualization of chromatin dynamics at locally

SUBMITTER: Raterink A 

PROVIDER: S-EPMC12407769 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature