Write and Read: Harnessing Synthetic DNA Modifications for Nanopore Sequencing.

ABSTRACT: An exciting feature of nanopore sequencing is its ability to record multi-omic information on the same sequenced DNA molecule. Well-trained models allow the detection of nucleotide-specific molecular signatures through changes in ionic current as DNA molecules translocate through the nanopore. Thus, naturally occurring DNA modifications, such as DNA methylation and hydroxymethylation, may be recorded simultaneously with the genetic sequence. Additional genomic information, such as chromatin state or the locations of bound transcription factors, may also be recorded if their locations are chemically encoded into the DNA. Here, we present a versatile "write-and-read" framework, where chemo-enzymatic DNA labeling with unnatural synthetic tags results in predictable electrical fingerprints in

SUBMITTER: Bertocchi U 

PROVIDER: S-EPMC12407796 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature