Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain.

ABSTRACT: Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.

SUBMITTER: Gaurivaud P 

PROVIDER: S-EPMC124079 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature