Ubiquitin degradation of the Ribonuclease H2 subunit B Rnh2B regulates the virulence of <i>Cryptococcus neoformans</i>.

ABSTRACT: The ubiquitin-proteasome system (UPS) regulates protein degradation in eukaryotes by polyubiquitinating substrate proteins, with F-box proteins serving as key components for substrate recognition. Research has shown that the absence of Cdc4, an F-box protein in C. neoformans, decreases virulence, yet the mechanisms by which Cdc4 affects these processes remain unclear. Using an iTRAQ-based proteomic strategy, we recognized Ribonuclease H2 subunit B (Rnh2B) as a target substrate for Cdc4. Protein-protein interaction assays have confirmed the interaction between Cdc4 and Rnh2B, indicating that Cdc4 regulates Rnh2B stability through ubiquitination. Functional analyses revealed that both the knockout and overexpression of RNH2B impair growth at 37°C, with the latter also affecting

SUBMITTER: Guo MR 

PROVIDER: S-EPMC12407989 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature