Epigenetic regulation of breast ductal carcinoma in situ by miR-217 through DNMT1 and Hedgehog-GLI pathway.

ABSTRACT: Ductal carcinoma in situ (DCIS) is a noninvasive precursor of breast cancer with a high potential for progression. Aberrant DNA methylation plays a pivotal role in early tumorigenesis, yet the regulatory mechanisms remain incompletely defined. Integrated bioinformatic analysis of methylation and transcriptomic datasets identified miR-217 as a candidate regulator of DNA methyltransferase 1 (DNMT1). Functional validation was conducted through RT-qPCR, dual-luciferase reporter assays, methylation-specific PCR, chromatin immunoprecipitation, and phenotypic assays in ZR-75-1 cells. An in vivo xenograft model using nude mice was employed to verify the regulatory axis. Expression of miR-217 was significantly reduced in DCIS tissues and inversely correlated with DNMT1 levels. Direct binding betwee

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12408178 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature