Discovery and early validation of serum protein signatures in untreated multiple sclerosis patients: identification of candidate biomarkers for diagnosis and stratification.

ABSTRACT:

Background

Despite progress in serum biomarker research, reliable tools for early diagnosis and patient stratification in multiple sclerosis (MS) remain limited. This study uses proteomic profiling in untreated MS patients to identify early disease-associated biomarkers.

Methods

We conducted an unbiased proteomic screen to capture broad serum protein expression profiles in a well-characterized discovery sample: 7 relapsing remitting MS (RRMS), 7 secondary progressive MS (SPMS), 4 with primary progressive MS (PPMS) alongside 6 healthy controls (HC). Twelve candidate biomarkers were subsequently validated by ELISA in an independent sample comprising 80 untreated MS patients (38 RRMS, 21 SPMS, 21 PPMS) and 21 age- and sex-matched HC from southern Spain.

Results

In the di

SUBMITTER: Brichette-Mieg I 

PROVIDER: S-EPMC12408279 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature