Flavonoid compound from <i>Forsythia suspensa</i> leaves inhibits adenovirus infection related to cell cycle based on UHPLC-Q-Exactive-Orbitrap/MS and experimental validation.

ABSTRACT:

Introduction

Forsythia suspensa leaves (FSL), a traditional Chinese ethnomedicinal herbal material used to prepare health-promoting infusions and pharmacologically noted for their robust anti-inflammatory, antioxidant, and broad-spectrum antiviral activities, nevertheless have an as-yet-uncharacterized molecular mechanism of action against human adenovirus (HAdV).

Methods

Ultra-high-performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive-Orbitrap/MS) was employed to identification of FSL components. Publicly available GEO datasets were mined to identify HAdV-associated differentially expressed genes (DEGs). An integrated analysis of GEO datasets and network pharmacology to predict the key molecular targets of FSL flavonoi

SUBMITTER: Wang L 

PROVIDER: S-EPMC12408589 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature