Integral approach to organelle profiling in human iPSC-derived cardiomyocytes enhances <i>in vitro</i> cardiac safety classification of known cardiotoxic compounds.

ABSTRACT:

Introduction

Efficient preclinical prediction of cardiovascular side effects poses a pivotal challenge for the pharmaceutical industry. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are becoming increasingly important in this field due to inaccessibility of human native cardiac tissue. Current preclinical hiPSC-CMs models focus on functional changes such as electrophysiological abnormalities, however other parameters, such as structural toxicity, remain less understood.

Methods

This study utilized hiPSC-CMs from three independent donors, cultured in serum-free conditions, and treated with a library of 17 small molecules with stratified cardiac side effects. High-content imaging (HCI) targeting ten subcellular organelles, combined with multi-electrode

SUBMITTER: Szabo BR 

PROVIDER: S-EPMC12408628 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature