Callus culture-derived regeneration and molecular characterization of regenerated <i>Stevia rebaudiana</i>: implications for steviol glycoside production and genetic stability.

ABSTRACT: The plant Stevia rebaudiana (Asteraceae) is gaining popularity as a zero-calorie natural sugar substitute. This paper investigates the regeneration of S. rebaudiana from callus, emphasizing steviol glycoside (SGs) production and the evaluation of genetic similarity. The highest rate of callus induction (89.20%) and maximum biomass were obtained from leaf explants using Murashige and Skoog (MS) medium, optimized with the addition of Naphthalene acetic acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4-D). MS medium containing NAA and 6-Benzylaminopurine (BAP) was most effective for shoot regeneration, yielding the highest shoot induction rate (87.77%) and robust plant growth. Rooting efficiency was significantly enhanced by using a quarter-strength MS medium with Indole-3-acet

SUBMITTER: Biswas P 

PROVIDER: S-EPMC12408629 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature