Rolipram and Electrical Stimulation Synergistically Promote Neuronal Differentiation of Adipose-derived Stromal Cells: an in Vitro Study.

ABSTRACT: This study investigates gold-decorated polycaprolactone/chitosan nanofibers as conductive scaffolds for promoting neuronal differentiation of adipose-derived mesenchymal stromal cells (ADSCs) harvested from the dorsal interscapular region of Wistar rats under electrical stimulation (ES) with optimal rolipram concentrations. The scaffold was fabricated through electrospinning and in situ synthesis of gold nanoparticles (AuNPs). Morphology and AuNPs distribution were evaluated using a Field Emission Scanning Electron Microscope (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Rolipram, known to increase neuronal cyclic adenosine monophosphate (cAMP) activity and reduce inflammation, was loaded into the scaffolds using alginate hydrogel. The scaffolds were subjected to a release study

SUBMITTER: Rahimzadegan M 

PROVIDER: S-EPMC12408716 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature