Tissue-Resident Mesenchymal Stem/stromal Cells (MSC) Modulate the Angiogenic Processes in Brain Arteriovenous Malformations (bAVM).

ABSTRACT:

Background

Mesenchymal stem/stromal cells (MSCs) have been mainly studied in the context of cell-based therapy for a variety of medical conditions, including cerebrovascular diseases. However, the role of tissue-resident MSCs in the pathophysiology of cerebrovascular diseases in general and of brain arteriovenous malformation (bAVM) in particular is currently unknown, and was investigated in this study.

Methods

Human bAVM tissues were used to identify MSCs in situ (n = 10) and to isolate them ex vivo (n = 3). The paracrine effects of bAVM-MSCs on endothelial cells (ECs) were assessed in an ex vivo model using MSC-derived supernatants (SNs) and the EC line HUVEC. Selected functional assays were validated using a second EC line (HCAEC).

Results

In situ, cells with a MSC

SUBMITTER: Dumitru CA 

PROVIDER: S-EPMC12408775 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature