High-Throughput Label-Free Continuous Quantification of Muscle Stem Cell Proliferation and Myogenic Differentiation.

ABSTRACT:

Background

Quantifying muscle satellite cell proliferation and differentiation is crucial for applications in muscle regeneration, disease modeling, and cultivated meat research. Traditional fluorescence-based assays, while sensitive, are labor-intensive, endpoint-restricted, and disruptive to myotube integrity.

Methods

In this study, we present a novel high-contrast brightfield (HCBF) imaging technique for high-throughput, label-free assessment of both satellite cell proliferation and myogenic differentiation. Using the BioTek Cytation 5 automated imager and Gen5 software (Agilent Technologies), we optimized imaging parameters to achieve continuous, highly time-resolved quantification in standard 96- and 384-well formats without any additional reagents or cell manipulation

SUBMITTER: Skrivergaard S 

PROVIDER: S-EPMC12408787 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature