Recellularization of decellularized vascular grafts via aligned seeding of endothelial cells derived from human iPS cells.

ABSTRACT: The development of decellularized vascular tissues for tissue engineering and vascular implants presents a promising approach to creating functional blood vessels. However, effective endothelialization with human endothelial cells remains challenging. This study examined the endothelialization of decellularized porcine aortas using human induced pluripotent stem (hiPS) cell-derived endothelial cells. Various decellularization methods were used to create vessels with different luminal surface properties. hiPS-derived endothelial cells seeded on these vessels showed adhesion and alignment, particularly on those decellularized with the high hydrostatic pressure (HHP) method. These cells expressed higher levels of EphrinB2 compared to those cultured on flat surfaces, suggesting they respond to

SUBMITTER: Kobayashi M 

PROVIDER: S-EPMC12408831 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature