Attenuation of IFITM proteins' antiviral activity through sequestration into intraluminal vesicles of late endosomes.

ABSTRACT:

Introduction

Interferon-induced transmembrane proteins (IFITMs) inhibit the entry of diverse enveloped viruses. The spectrum of antiviral activity of IFITMs is largely determined by their subcellular localization. IFITM1 localizes to and primarily blocks viral fusion at the plasma membrane, while IFITM3 prevents viral fusion in late endosomes by accumulating in these compartments. We and others have previously shown that cyclosporine treatment relieves the fusion block for the Influenza A virus, but the mechanism of this rescue remained unclear.

Results

Here, we report the existence of at least two distinct pools of IFITMs in cyclosporine treated cells. Major pools of IFITM1 and IFITM3 were found in endosomes, with IFITM1 relocating from the plasma membrane by a mechanism in

SUBMITTER: Prikryl D 

PROVIDER: S-EPMC12409171 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature