Variants of the Ebola virus matrix protein VP40 have differential effects on oligomerization and plasma membrane interactions.

ABSTRACT: The Ebola virus (EBOV) has been responsible for several outbreaks in Africa over the last decade causing severe hemorrhagic fever with limited treatment options. EBOV is a lipid-enveloped filamentous negative-sense RNA virus with a genome encoding seven proteins. This includes the matrix protein VP40, which regulates assembly and budding of new virions from the inner leaflet of the host cell plasma membrane (PM). VP40 is a multifaceted protein that has different oligomeric states to regulate different parts of the virus life cycle. We investigated how two patient-derived mutations of VP40 samples (R204H and H269R) altered VP40 electrostatics, VP40 oligomeric state, interactions with PM anionic lipids and how these changes affect virus-like particle formation using both laboratory and compu

SUBMITTER: Motsa BB 

PROVIDER: S-EPMC12409429 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature