Supplement C18:1 in Culture Medium to Improve Survival Rate and Fermentation Activity of Lyophilization of <i>Lacticaseibacillus paracasei</i> L9.

ABSTRACT: This study explored the impact of C18:1 fatty acid supplementation in culture medium on the survival rate, fermentation activity, and membrane stability of Lacticaseibacillus paracasei L9 during freeze-drying. Cells cultured in medium supplemented with C18:1 (0.2 mg/ml) exhibited a higher survival rate (95.38%) after freeze-drying compared to the control (83.56%). The fermentation activity of the freeze-dried powder was significantly enhanced, with improvements in viable count (Δ viable counts: 1.79 Log CFU/ml), pH reduction (Δ pH: 2.03), and titratable acidity (Δ titratable acidity: 99.4 °T) in skim milk. Supplementation with C18:1 increased the activity of key enzymes, such as β-galactosidase (13.25 U/ml) and lactate dehydrogenase (2.16 U), while reducing cell membrane perm

SUBMITTER: Miao W 

PROVIDER: S-EPMC12409430 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature