Cysteine oxidation of a redox hub within complex I can facilitate electron transport chain supercomplex formation.

ABSTRACT: The mitochondrial electron transport chain (ETC) is a four complex unit that could be considered the most essential infrastructure within the mitochondria, as it primarily functions to generate the mitochondrial membrane potential (ΔΨm), which can then be utilized for ATP synthesis or heat production. Another important aspect of ETC function is the generation of mitochondrial reactive oxygen species (mtROS), which are essential physiologic signaling mediators that can be toxic to the cell if their levels become too high. Currently, it remains unresolved how a highly utilized and functioning ETC can sense excessive mtROS generation and adapt, to enhance ΔΨm. Here, we identified a redox hub consisting of cysteine (Cys) residues 64, 75, 78, and 92 within Ndufs1 of complex I of the ETC. Oxidat

SUBMITTER: Chen R 

PROVIDER: S-EPMC12409452 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature