Effect of Ipomoeassin F on the Synthesis of Membrane and Secretory Proteins in Triple-Negative Breast Cancer Cells.

ABSTRACT: Ipomoeassin F (Ipom-F) is a natural compound that exhibits a potent cytotoxic effect on triple-negative breast cancer (TNBC) cells. The mechanism underlying this selective potency remains unclear. To elucidate this mechanism, we analyzed the proteome profiles of the TNBC MDA-MB-231 cells after exposure to Ipom-F at different time points and increasing doses using a quantitative proteomic method. Our proteomic data demonstrate that the major effect of Ipom-F on MDA-MB-231 cells is the inhibition of membrane and secreted protein expression. These findings align with the recently uncovered molecular mechanism of action of Ipom-F, which binds to Sec61-α and inhibits the cotranslational import of proteins into the endoplasmic reticulum. We have defined a subset of membrane and secreted proteins

SUBMITTER: Sicairos B 

PROVIDER: S-EPMC12409539 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature